ABSTRACT
Cancer is the uncontrolled growth of abnormal cells in the body. Symptoms of cancer depend on the type and location of the cancer. Paclitaxel obtained from Taxus brevifolia is an effective natural drug used in cancer treatment, so there arises a need of its estimation in biological fluids for the pharmacokinetic studies in clinical trials and in various pharmaceutical dosage forms including nanoparticles, cremophore based formulations and paclitaxel releasing stents. Literature survey reveals many chromatographic methods for estimation of paclitaxel by HPLC, LC-MS-MS and UPLC to get very accurate, precise and robust method with lower run time and higher recovery. An important step in the development of liposome-based formulations is estimation of the free drug concentration in the aqueous solution surrounding liposomes